5-Amino-1MQ
5-Amino-1MQ
A selective, cell-permeable inhibitor of NNMT, studied for fat metabolism, lean-mass preservation, and intracellular NAD+ support via SIRT1 activation.
Overview
5-Amino-1MQ is a selective, cell-permeable inhibitor of nicotinamide N-methyltransferase (NNMT), studied for its potential to support fat metabolism, preserve lean muscle mass, and elevate intracellular NAD+ levels by blocking NNMT and activating SIRT1 pathways. It is not FDA-approved for any indication.
Preclinical models demonstrate reductions in fat mass and improvements in metabolic markers, though human clinical data remain limited to early investigational work. This protocol presents a once- or twice-daily subcutaneous approach using practical dilution for clear insulin-syringe measurements.
At a Glance
Protocol
Tolerance assessment followed by standard daily dosing; twice-daily option for sustained NNMT inhibition.
Inject once or twice daily subcutaneously. Due to the compound's plasma half-life of approximately 3.8–6.9 hours, twice-daily dosing may provide more sustained NNMT inhibition. At standard doses, a single vial provides only a few days of material, so plan multiple vials for extended protocols. Dosing extrapolates from preclinical models; human clinical validation remains limited.
Dose progression
Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.
Videos
How 5-Amino-1MQ works.
5-Amino-1MQ selectively inhibits NNMT, a cytosolic enzyme that methylates nicotinamide using S-adenosylmethionine (SAM) as a methyl donor. In obesity and metabolic dysfunction, NNMT is overexpressed in adipose tissue, depleting nicotinamide and reducing NAD+ availability.
By inhibiting NNMT, 5-Amino-1MQ spares nicotinamide for NAD+ synthesis via the salvage pathway, activating SIRT1 pathways associated with mitochondrial biogenesis, fat oxidation, and metabolic flexibility. Preclinical studies have demonstrated reductions in body weight and fat mass without affecting food intake, along with preserved or improved lean muscle mass. However, large-scale controlled human efficacy data remain unavailable.
Effects
Observations from clinical or preclinical literature.
Caution
Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.
CoFactors
Life Factors
Complementary strategies for best outcomes.
Metrics
Day-to-day metrics worth tracking through the protocol.
- Body composition (weight, waist circumference, body fat %) — monitor changes to gauge metabolic response
- Energy levels and exercise performance — track daily to identify improvements in endurance and recovery
- Injection-site reactions — note any redness, stinging, or discomfort to guide site rotation
- Subjective well-being and appetite — monitor for any unexpected changes during the protocol
Labs
Baseline and periodic bloodwork to monitor systemic health during the protocol.
Supplies Calculator
Estimates assume the schedule defined for this peptide.
Dose Calculator
Dose Calculator
Preparation
Careful technique preserves potency. Solution should be clear — do not shake.
- Allow vial to reach room temperature for 15–20 minutes before reconstitution.
- Draw the chosen bacteriostatic water volume with a sterile syringe.
- Inject slowly down vial wall; avoid foaming.
- Gently swirl/roll until dissolved (do not shake).
- Label with reconstitution date and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.
- Use within 30 days; discard any unused solution after 30 days.
